Next-Generation Sequencing of Infectious Pathogens

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Strategies and Clinical Applications of Next Generation Sequencing

Abstract DNA sequencing is one of the great valuable techniques in molecular biology, which can be used to detect the sequence of nucleotides in a DNA fragment. The high-throughput se­quencing known as Next Generation Sequencing (NGS) revolutionized genomic research and molecular biology; therefore, the whole human genome can be sequenced with a low cost in several days. NGS technology is simi...

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Strategies and Clinical Applications of Next Generation Sequencing

Abstract DNA sequencing is one of the great valuable techniques in molecular biology, which can be used to detect the sequence of nucleotides in a DNA fragment. The high-throughput se­quencing known as Next Generation Sequencing (NGS) revolutionized genomic research and molecular biology; therefore, the whole human genome can be sequenced with a low cost in several days. NGS technology is simi...

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Understanding pathogens in the era of next generation sequencing.

What is a pathogen? Medical textbooks usually define a pathogen as any microorganism that causes disease. However, this widespread definition is problematic on a number of counts [1, 11]. Moreover, a generally accepted definition is not forthcoming among medical microbiologists, immunologists, and physicians. Here it is argued that there is another, and more pressing question to be asked, namel...

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Next-Generation Sequencing

Next-generation sequencing (NGS), otherwise known as deep or massively parallel sequencing, refers to the technological advances in DNA sequencing instrumentation that enable the generation of hundreds of thousands to millions of sequence reads per run. Sequencing of the human genome, which was once a >10-year endeavor by the NIH at the cost of approximately $3 billion (1), can now be done rout...

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Next-Generation Sequencing

1Human Genetics Center, The University of Texas School of Public Health, Houston, TX 77025, USA 2Departments of Biomedical Informatics and Psychiatry and Vanderbilt-Ingram Cancer Center (VICC), Vanderbilt University Medical Center, Nashville, TN 37232, USA 3Department of Genetics, The University of Georgia, Life Sciences Building, Athens, GA 30602, USA 4Human Genome Sequencing Center, Baylor Co...

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ژورنال

عنوان ژورنال: JAMA

سال: 2019

ISSN: 0098-7484

DOI: 10.1001/jama.2018.21669